Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection

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• 作者：Aditya Sharma ; Zimple Matharu ; G. Sumana ; Pratima R. Solanki ; C.G. Kim ; B.D. Malhotra
• 关键词：Aflatoxin ; Cysteamine ; Gold nanoparticles ; Immunosensor ; Self assembled monolayer
• 刊名：Thin Solid Films
• 出版年：2010
• 出版时间：30 November 2010
• 年：2010
• 卷：519
• 期：3
• 页码：1213-1218
• 全文大小：845 K

文摘

Aflatoxin B₁ antibody (aAFB₁) covalently attached to cysteamine functionalized-gold nanoparticles (C-AuNP) has been immobilized onto 4-mercaptobenzoic acid (MBA) based self assembled monolayer (SAM) on gold electrode (MBA/Au), for the fabrication of BSA/aAFB₁-C-AuNP/MBA/Au immunoelectrode. This immunoelectrode has been characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and electrochemical characterization techniques. The electrochemical response studies reveal that the BSA/aAFB₁-C-AuNP/MBA/Au immunoelectrode can be used to detect AFB₁ in the range of 10–100 ng dL^− 1 and has sensitivity as 0.45 μA ng^− 1 dL, limit of detection as 17.90 ng dL^− 1 and a response time of 60 s.