Molecular cloning and transcriptional analysis of WRKY and solavetivone biosynthetic genes in the hairy roots of Hyoscyamus albus
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- 作者：Moriyuki Kawauchi1 ; mkawauchi1987@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Toshi-Hide Arima ; arima@pu-hiroshima.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Masanori Kuroyanagi2 ; kuroyang@u-shizuoka-ken.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：CuSO4 ; copper sulfate ; DDBJ ; DNA Data Bank of Japan ; Dof ; DNA binding with one finger ; hahpo1 ; Hyoscyamus albus Hyoscyamus premnaspirodiene oxygenase 1 ; HAHR ; hairy roots of Hyoscyamus albus ; hatps1 ; Hyoscyamus albus terpene synthase 1 ; hawrky1 ; Hyoscyamus albus wrky transcription factor 1 ; MEGA6 ; Molecular Evolutionary Genetic Analysis 6 ; MeJA ; methyl jasmonate ; MS ; Murashige and Skoog ; MYB ; myeloblastosis ; NCBI ; National Center for Biotechnology Information ; PCR ; polymerase chain reaction ; RAC
- 刊名：Plant Gene
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：5
- 期：Complete
- 页码：78-86
- 全文大小：2111 K
文摘
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The premnaspirodiene synthase hatps1 was cloned from Hyoscyamus albus hairy roots.
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10">The premnaspirodiene oxygenase hahpo1 was cloned from Hyoscyamus albus hairy roots.
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15">The hatps1 and hahpo1 promoters contain W-boxes, which are WRKY cis-elements.
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The class IIa WRKY hawrky1 was cloned from Hyoscyamus albus hairy roots.
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The induction pattern of hawrky1 matched that of hahpo1.