Differences in the Phosphorylation-Dependent Regulation of Prenylation of Rap1A and Rap1B

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• 作者：Jessica M. Wilson¹ ; Jeremy W. Prokop² ; Ellen Lorimer¹ ; Elizabeth Ntantie³ ; Carol L. Williams¹ ; ^{williams@mcw.edu}
• 关键词：PBR ; polybasic region ; GEF ; guanine nucleotide exchange factor ; PKA ; protein kinase A ; Ctx ; cholera toxin ; GPCR ; Gαs protein-coupled receptor ; NECA ; 5&prime ; -N-ethylcarboxamidoadenosine ; Iso ; isoproterenol ; GFP ; green fluorescent protein
• 刊名：Journal of Molecular Biology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：428
• 期：24
• 页码：4929-4945
• 全文大小：2911 K
• 卷排序：428

文摘

Structural and evolutionarily conserved differences in Rap1A and Rap1B are examined. C-terminal polybasic region differences regulate binding to SmgGDS and prenylation. Phosphorylation of serine 180 does not inhibit prenylation of Rap1A, unlike Rap1B. GPCR activation inhibits Rap1A prenylation independently of S-180 phosphorylation.