Protocol for culturing low density pure rat hippocampal neurons supported by mature mixed neuron cultures

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• 作者：Qian Yang ; Yini Ke ; Jianhong Luo ; Yang Tang ; ^{tyx90@zju.edu.cn}
• 关键词：Primary neuron culture ; Protocol ; Morphology ; Neurotrophic support
• 刊名：Journal of Neuroscience Methods
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：277
• 期：Complete
• 页码：38-45
• 全文大小：3396 K
• 卷排序：277

文摘

A method for culturing very low density rat hippocampal neurons is described. Low density culture depend on neurotrophic support from mature hippocampal culture. This method yields neurons for good morphology and electrophysiology analysis.