Long noncoding RNA expression patterns in lymph node metastasis in colorectal cancer by microarray

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• 作者：Qiang Rui ; Zipeng Xu ; Peng Yang ; Zhenyu He
• 关键词：Lymph node metastasis ; Colorectal cancer ; Long noncoding RNA ; Microarray
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：75
• 期：Complete
• 页码：12-18
• 全文大小：1793 K

文摘

To profile the long noncoding RNA (lncRNA) expression patterns in normal lymph node (NLN) of colorectal cancer compared with paired metastatic lymph node (MLN).

Materials and methods

MLN, NLN lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in three patients; quantitative reverse transcription–polymerase chain reaction validation of 3 differentially expressed lncRNAs was conducted in another 26 patients. The lncRNAs’ functions were predicted through coexpressed mRNA annotations.

Result(s)

A total of 1133 lncRNA transcripts transcripts were dysregulated in metastatic lymph node, compared with normal lymph node. The expressions of the 3 chosen lncRNAs were validated by quantitative reverse transcription–polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to colorectal cancer by cis- and/or trans-regulation of protein-coding genes.

Conclusion(s)

This study constitutes the first report of lncRNA expression patterns in human MLN, NLN of colorectal cancer. More than 1000 dysregulated lncRNA transcripts are found by microarray.