Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis

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• 作者：Marie Okumura¹ ; Yoshiko Fujitani¹ ; Masahiko Maekawa¹ ; Jittima Charoenpanich² ; Hunja Murage³ ; Kazuhide Kimbara¹ ; ⁴ ; Nurettin Sahin⁵ ; Akio Tani¹ ; ^{atani@okayama-u.ac.jp}
• 关键词：Methylobacterium species ; Rice seeds ; Whole-cell matrix-assisted laser desorption/ionization-mass spectrometry
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：123
• 期：2
• 页码：190-196
• 全文大小：734 K
• 卷排序：123

文摘

Methylobacterium species are methylotrophic bacteria that widely inhabit plant surfaces. In addition to studies on methylotrophs as model organisms, research has also been conducted on their mechanism of plant growth promotion as well as the species–species specificity of plant–microbe interaction. We employed whole-cell matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (WC-MS) analysis, which enables the rapid and accurate identification of bacteria at the species level, to identify Methylobacterium isolates collected from the rice seeds of different cultivars harvested in Japan, Thailand, and Kenya. Rice seeds obtained from diverse geographical locations showed different communities of Methylobacterium species. We found that M. fujisawaense, M. aquaticum, M. platani, and M. radiotolerans are the most frequently isolated species, but none were isolated as common species from 18 seed samples due to the highly biased communities in some samples. These findings will contribute to the development of formulations containing selected species that promote rice growth, though it may be necessary to customize the formulations depending on the cultivars and farm conditions.