A highly conserved epitope-vaccine candidate against varicella-zoster virus induces neutralizing antibodies in mice

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• 作者：Rui Zhu^b ; ¹ ; Jian Liu^a ; ¹ ; Chunye Chen^b ; Xiangzhong Ye^c ; Longfa Xu^b ; Wei Wang^a ; Qinjian Zhao^b ; Hua Zhu^d ; Tong Cheng^a ; ^b ; ^{tcheng@xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Ningshao Xia^a ; ^b
• 关键词：Varicella-zoster virus ; Neutralizing linear epitope ; Epitope vaccine ; Glycoprotein E
• 刊名：Vaccine
• 出版年：2016
• 出版时间：18 March 2016
• 年：2016
• 卷：34
• 期：13
• 页码：1589-1596
• 全文大小：2454 K

文摘

Varicella-zoster virus (VZV) is a highly infectious agent of varicella and herpes zoster (HZ). Vaccination is by far the most effective way to prevent these diseases. More safe, stable and efficient vaccines, such as epitope-based vaccines, now have been increasingly investigated by many researchers. However, only a few VZV neutralizing epitopes have been identified to date. We have previously identified a linear epitope between amino acid residues 121 and 135 of gE. In this study, we validated that this epitope is highly conserved amongst different VZV strains that covered five existing phylogenetic clades with an identity of 100%. We evaluated the immunogenicity of the recombinant hepatitis B virus core (HBc) virus-like particles (VLPs) which included amino acids (121–135). VZV-gE-specific antibodies were detected in immunized mouse serum using ELISA. The anti-peptide antiserum positively detected VZV via Western blot and immunofluorescent staining assays. More importantly, these peptides could neutralize VZV, indicating that these peptides represented neutralizing epitopes. These findings have important implications for the development of epitope-based protective VZV vaccines.