Intracellular delivery of recombinant proteins via gold nanoparticle-DNA aptamer composites is independent of the protein physicochemical properties and cell type

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• 作者：Ji-Hyun Yeom^a ; ¹ ; Minju Joo^a ; ¹ ; Boeun Lee^a ; Keun P. Kim^a ; Nam-Chul Ha^b ; Yoonkyung Park^c ; Jeehyeon Bae^d ; ^{jeehyeon@cau.ac.kr} ; Kangseok Lee^a ; ^{kangseok@cau.ac.kr}
• 关键词：DNA aptamer ; Gold nanoparticle ; Protein delivery ; Intravenous delivery
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：45
• 期：Complete
• 页码：5-10
• 全文大小：2121 K
• 卷排序：45

文摘

Here, we report that the gold nanoparticle–DNA aptamer (AuNP–Apt) conjugate-based system can efficiently deliver recombinant proteins into mammalian cells in a manner independent of their size, isoelectric point, and cellular localization. Additionally, AuNP–Apt system-assisted protein delivery can be effective on primary and stem cells, indicating that its use is not limited to fast-dividing cells. We further show that the intravenously administered AuNP–Apt system can deliver proteins into rat organs. Our findings show that this system can serve as a simple, efficient, and versatile platform for the delivery of recombinant proteins into mammalian living systems.