Dynamic in the concentration of whey proteins in the mammary secretion of goats during the dry period

详细信息    查看全文

• 作者：R.F.S. Raimondo^a ; ^{rfraimondo@gmail.com} ; F.B. Brandespim^a ; ¹ ; ^{brandespin65@yahoo.com.br} ; A.P.M. Prina^a ; ¹ ; S.I. Miyashiro^a ; ¹ ; J.P.E. Saut^b ; ² ; ^{jpsaut@famev.ufu.br} ; C.S. Mori^a ; ¹ ; F.C. Pogliani^a ; ¹ ; ^{pogliani@yahoo.com} ; E.H. Birgel Junior^c ; ³ ; ^{ehbirgel@usp.br}
• 关键词：Saanen goat ; SDS-PAGE ; Lactoferrin ; Albumin ; Immunoglobulin ; Degradation of casein
• 刊名：Small Ruminant Research
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：113
• 期：1
• 页码：239-246
• 全文大小：636 K

文摘

To study the dynamics on the concentration of mammary secretion whey proteins of goats during the dry period eight Saanen goats were selected through traditional methods of semiology. The mammary secretion was assessed eight times during the 30-day dry period. Whey was obtained by coagulating mammary secretion with rennet. Total proteins concentration was determined by the biuret method and protein fractions were determined by polyacrylamide gel electrophoresis on a 12 % gel (SDS-PAGE 12 % ). There was an increase in protein concentration of mammary secretion serum proteins during the dry period with changes in the percentage distribution of proteins fractions, accompanied with the appearance of new fractions. It may be concluded that involution is associated with increased concentration of components of the innate (albumin, lactoferrin) and acquired (immunoglobulin) system and with evidences for increased degradation of casein.