Revisiting 1,3,4-Oxadiazol-2-ones: Utilization in the Development of ABHD6 Inhibitors

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• 作者：Jayendra Z. Patel^a ; ^{jayendra.patel@uef.fi" class="auth_mail" title="E-mail the corresponding author} ; ^{jayorgchem137@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; John van Bruchem^a ; Tuomo Laitinen^a ; Agnieszka A. Kaczor^a ; ^b ; Dina Navia-Paldanius^c ; Teija Parkkari^a ; Juha R. Savinainen^c ; Jarmo T. Laitinen^c ; Tapio J. Nevalainen^a
• 关键词：2-AG ; 2-arachidonoylglycerol ; hABHD6 ; human recombinant 伪/尾 hydrolase domain 6 ; hMAGL ; human recombinant monoacylglycerol lipase ; hFAAH ; human recombinant fatty acid amide hydrolase ; hABHD12 ; human recombinant 伪/尾 hydrolase domain 12 ; WWL70 ; N-methyl-N-[[3-(4-pyridinyl)phenyl]-methyl]-4&prime ; -(aminocarbonyl)[1 ; 1&prime ; -biphenyl]-4-yl carbamic acid ester ; JZP-327A ; S-3-(1-(4-isobutylphenyl)ethyl)-5-methoxy-1 ; 3 ; 4-oxadiazol-2(3H)-one ; JZP-430 ; 4-morpholino-1 ; 2 ; 5-thiadiazol-3-yl cyclooctyl(methyl)c
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2015
• 出版时间：1 October 2015
• 年：2015
• 卷：23
• 期：19
• 页码：6335-6345
• 全文大小：1071 K

文摘

This article describes our systematic approach to exploring the utility of the 1,3,4-oxadiazol-2-one scaffold in the development of ABHD6 inhibitors. Compound 3-(3-aminobenzyl)-5-methoxy-1,3,4-oxadiazol-2(3H)-one (JZP-169, 52) was identified as a potent inhibitor of hABHD6, with an IC₅₀ value of 216 nM. This compound at 10 渭M concentration did not inhibit any other endocannabinoid hydrolases, such as FAAH, MAGL and ABHD12, or bind to the cannabinoid receptors (CB₁ and CB₂). Moreover, in competitive activity-based protein profiling (ABPP), compound 52 (JZP-169) at 10 渭M selectively targeted ABHD6 of the serine hydrolases of mouse brain membrane proteome. Reversibility studies indicated that compound 52 inhibited hABHD6 in an irreversible manner. Finally, homology modelling and molecular docking studies were used to gain insights into the binding of compound 52 to the active site of hABHD6.