Synthesis of stable isotopically labeled peptides with filter-assisted enzymatic labeling for the diagnosis of hepatitis B virus infection utilizing mass spectrometry-based proteomics strategy

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• 作者：Hsing-Fen Tsai ; He-Hsuan Hsiao ; ^{hhhsiao@dragon.nchu.edu.tw}
• 关键词：Filter-assisted enzymatic labeling ; Hepatitis B virus ; Mass spectrometry ; Parallel reaction monitoring ; Stable isotopically labeled peptides
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：956
• 期：Complete
• 页码：32-39
• 全文大小：1164 K
• 卷排序：956

文摘

Facile synthesis of an inexpensive and highly reproducible stable isotopically labeled peptides. Complete incorporation of two 18O atoms into synthesized peptides with filter-assisted enzymatic labeling. Targeted analysis with parallel reaction monitoring assay for the disease diagnosis.