An insight into fusion technology aiding efficient recombinant protein production for functional proteomics
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- 作者：Dinesh K. Yadava ; 1 ; Neelam Yadavb ; 1 ; 2 ; Sarika Yadavc ; Shafiul Haqued ; Narendra Tutejae ; ntuteja@amity.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Affinity tags ; Fusion protein ; Proteases ; Recombinant protein ; Solubility enhancing tag ; SUMO ; Tag removal ; Tandem affinity purification
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：612
- 期：Complete
- 页码：57-77
- 全文大小：1346 K
文摘
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Critical evaluation of fusion tags for enhanced production of recombinant proteins.
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Evaluation of combinatorial tags for enhanced solubility and affinity purification.
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Critical analysis of tag removal strategies to recover tag free target protein.
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Guided selection of suitable fusion tag for successful production of novel proteins.