A simple method for large-scale purification of nucleic acids from plants using calcium phosphate-type monetite

详细信息    查看全文

• 作者：Jaouad Anissi^a ; ^b ; Mohammed El Hassouni^b ; Abdelkrim Ouardaoui^a ; Khalid Sendide^a ; ^{k.sendide@aui.ma}
• 关键词：Calcium phosphate-type monetite ; Ultrasound-assisted synthesis ; Nucleic acids purification ; Plant DNA purification
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：52
• 期：Complete
• 页码：259-266
• 全文大小：2065 K
• 卷排序：52

文摘

Synthesis of a solid support of Calcium Phosphate used for nucleic acids purification. The synthesis process was optimized based on the change in the binding capacity. The process of binding and elution was optimized for high purification of nucleic acids. The high binding capacity of the solid support could allow economic purifications up to g of nucleic acids. The separation of nucleic acids can be performed from any environmental source.