Identification and characterization of a chitin deacetylase from a metagenomic library of deep-sea sediments of the Arctic Ocean
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- 作者：Jinlin Liu1 ; Zhijuan Jia1 ; Sha Li ; Yan Li ; Qiang You ; Chunyan Zhang ; Xiaotong Zheng ; Guomei Xiong ; Jin Zhao ; Chao Qi ; qichao@mail.ccnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jihong Yang ; cdcyang@mail.ccnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：rCdaYJ ; recombinant CdaYJ ; CDA ; chitin deacetylase ; EDTA ; ethylene diamine tetraacetic acid ; CTAB ; cetyltrimethyl ammonium bromide ; IPTG ; isopropyl β-d-1-thiogalactopyranoside ; X-Gal ; 5-bromo-4-chloro-3-indolyl-β-d- galacto-pyranoside ; ORF ; open reading frame ; NCBI ; national center of biotechnology information ; BLAST ; basic local alignment search tool ; MEGA ; Molecular Evolutionary Genetics Analysis ; PCR ; polymerase chain reaction ; OD ; optical density ; SDS-PAGE ; sodium dodecyl sulphate-polyacryla
- 刊名：Gene
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：590
- 期：1
- 页码：79-84
- 全文大小：368 K
文摘
- 5">
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5">A metagenomic library with 2750 clones was constructed from the Arctic deep-sea sediments.
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Sequencing of selected clones revealed many interesting genes.
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5">A chitin deacetylase gene cdaYJ was identified from the metagenomic library.
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Recombinant CdaYJ produced in Escherichia coli exhibited deacetylation activity.
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5">rCdaYJ showed highest deacetylation activity under slight alkaline and low temperature.
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