Recent Development of Droplet Microfluidics in Digital Polymerase Chain Reaction

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• 作者：Yi-Qiang FAN ; ^{fanyq@mali.buct.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Mei WANG ; Feng GAO ; Jian ZHUANG ; Gang TANG ; Ya-Jun ZHANG
• 关键词：Digital polymerase chain reaction ; Droplet microfluidics ; Genetic testing ; Gene sequencing ; Review
• 刊名：Chinese Journal of Analytical Chemistry
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：44
• 期：8
• 页码：1300-1307
• 全文大小：1330 K

文摘

Digital polymerase chain reaction (PCR) has been experiencing a rapid development during the past few years. Compared with the traditional qPCR, the accuracy of digital PCR for the absolute quantification of target gene has been significantly improved with the same primer and probe as qPCR. The development of digital PCR is directly related to the development of microfluidics. The integrated fluid circuit, which has a complicated fabrication process with high cost, is an early integration of the microfluidics and digital PCR. Recently, researchers are trying to apply the droplet microfluidics in digital PCR. A droplet microfluidic chip is able to generate millions of droplets within a short time. Each of these droplets containing no more than one target gene is a reaction chamber during the amplification process. After amplification, each droplet is tested to obtain the absolute quantification of the target gene. This paper reviews the recent progress of droplet digital PCR and the application of droplet digital PCR in biological, medical and environmental area.