Phenylmethanesulfonyl fluoride pretreatment stabilizes plasma lipidome in lipidomic and metabolomic analysis
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- 作者：Xiaolin Wang ; Xiao Gu ; Huahua Song ; Qingxiang Song ; Xiaoling Gao ; shellygao1@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yang Lu ; luyangssmu@163.com" class="auth_mail" title="E-mail the corresponding author ; Hongzhuan Chen ; hongzhuan_chen@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lipidomics ; Metabolomics ; Plasma lipidome stability ; Hepatic lipase and esterase ; Phenylmethanesulfonyl fluoride pretreatment ; Mass spectrometry
- 刊名：Analytica Chimica Acta
- 出版年：2015
- 出版时间：17 September 2015
- 年：2015
- 卷：893
- 期：Complete
- 页码：77-83
- 全文大小：1759 K
文摘
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An LC-MS lipidomic method for detecting diverse lipids in plasma was established.
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Bench-top stability of lipidome in mouse, rat and human plasma was evaluated.
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There was extensive lipid hydrolysis in mouse and rat but not in human plasma.
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Hepatic lipase and esterase were thought as causes of plasma lipidome instability.
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PMSF pretreatment could enhance plasma lipidome stability.