Dual-component gene detection for H7N9 virus - The combination of optical trapping and bead-based fluorescence assay
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- 作者：Di Cao1 ; Cheng-Yu Li1 ; Ya-Feng Kang ; Yi Lin ; Ran Cui ; Dai-Wen Pang ; Hong-Wu Tang ; hwtang@whu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Optical trapping ; Bead-based assay ; Quantum dots ; Dual-component detection ; H7N9 virus
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：1031-1037
- 全文大小：1115 K
文摘
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A dual-component virus gene detection was achieved by using optical trap and beads assay.
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This strategy employs size-distinct beads, biotinylated DNA and streptavidin coated quantum dots.
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Stable optical trapping and photostable QDs guarantee the stable detection condition.
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High sensitivity with economy was achieved due to the use of a low-cost tiny-power laser.