Quantitative detection of periodontopathic bacteria in lower respiratory tract specimens by real-time PCR

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• 作者：Kentaro Nagaoka^a ; ^b ; ^d ; Katsunori Yanagihara^a ; ^b ; ^{k-yanagi@net.nagasaki-u.ac.jp} ; Yosuke Harada^a ; ^b ; Koichi Yamada^a ; ^b ; Yohei Migiyama^a ; ^b ; Yoshitomo Morinaga^a ; ^b ; Koichi Izumikawa^b ; Shigeru Kohno^b ; ^c
• 关键词：Pneumonia ; Anaerobe ; Quantitative PCR ; Fusobacterium nucleatum ; Prevotella intermedia ; Porphyromonas gingivalis
• 刊名：Journal of Infection and Chemotherapy
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：23
• 期：2
• 页码：69-73
• 全文大小：279 K
• 卷排序：23

文摘

The presence of common periodontopathic bacteria, the Fusobacterium nucleatum-periodonticum-simiae group, Prevotella intermedia, and Porphyromonas gingivalis was determined from respiratory tract specimens of bacterial pneumonia by real-time PCR using universal and species-specific TaqMan probe/primer sets. 42 patients with infectious pneumonia and 45 patients without infectious pneumonia were retrospectively enrolled in clinical studies. Periodontopathic bacterial DNA was found in 57.1% cases of infectious pneumonia and 31.1% cases of noninfectious pulmonary disease. However, the proportion of periodontopathic bacterial DNA did not differ between the two groups, and the presence or proportion of periodontopathic bacterial DNA was not related to any clinical index of pneumonia. Only two pneumonia cases consisted of >30% Fusobacterium DNA, suggesting that Fusobacterium was the causal pathogen in these cases. Our findings suggest that the periodontopathic bacteria rarely proliferate and be etiological pathogen in lower airway tract. However, further study is necessary, focusing on the pathogenicity of F. nucleatum in pulmonary infectious disease.