Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii colonization

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• 作者：P. Blanco-Lobo^a ; V. Gonzá ; lez-Galá ; n^a ; ^b ; M. Garcí ; a-Quintanilla^a ; R. Valencia^b ; A. Cazalla^b ; C. Martí ; n^c ; I. Alonso^c ; P. Pé ; rez-Romero^a ; ^b ; J.M. Cisneros^a ; ^b ; J. Aznar^a ; ^b ; M.J. McConnell^a ; ^{mcconnell.mike75@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Acinetobacter baumannii ; Active surveillance ; Real-time PCR ; Colonization
• 刊名：Journal of Hospital Infection
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：94
• 期：1
• 页码：68-71
• 全文大小：257 K

文摘

Real-time polymerase chain reaction (PCR)-based approaches have not been assessed in terms of their ability to detect patients colonized by Acinetobacter baumannii during active surveillance. This prospective, double-blind study demonstrated that a real-time PCR assay had high sensitivity (100%) and specificity (91.2%) compared with conventional culture for detecting A. baumannii in 397 active surveillance samples, and provided results within 3 h. Receiver-operator curve analyses demonstrated that the technique has diagnostic accuracy of 97.7% (95% confidence interval 96.0–99.3%). This method could facilitate the rapid implementation of infection control measures for preventing the transmission of A. baumannii.