Coding potential of UL/b鈥?from the initial source of rhesus cytomegalovirus Strain 68-1
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- 作者：Rachel B. Gill ; J. Jason Bowman ; Tammy Krogmann ; Kurt Wollenberg ; David M. Asher ; Jeffrey I. Cohen
- 关键词：Human cytomegalovirus ; Rhesus cytomegalovirus ; Cercopithecine herpesvirus ; macacine herpesvirus 3 ; Cynomolgus macaque cytomegalovirus ; UL/b&prime
- 刊名：Virology
- 出版年：December, 2013
- 年：2013
- 卷：447
- 期：1-2
- 页码：208-212
- 全文大小：410 K
文摘
Rhesus cytomegalovirus (RhCMV) 68-1 is the prototypic strain of RhCMV that has been used for pathogenesis and vaccine development. We determined the complete sequence of the RhCMV 68-1 UL/b鈥?region directly from the original urine from which RhCMV 68-1 was isolated in 1968, and compared it to other RhCMVs. The laboratory passaged RhCMV 68-1 has inversions, deletions, and stop codons in UL/b鈥?that are absent in the original isolate and other low passage RhCMV isolates. Fourteen of the 17 open reading frames (ORFs) in 68-1 UL/b鈥?in the original isolate share >95% amino acid identity with low passage RhCMV. The original isolate retains 6 ORFs that encode 伪-chemokine-like proteins, including RhUL146 and RhUL146b that share only 92% and 81% amino acid identity, respectively, with a contemporary low passage RhCMV isolate. Identification of the original RhCMV 68-1 UL/b鈥?sequence is important for using RhCMV 68-1 in pathogenesis and vaccine studies.