Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate

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• 作者：Xin Sui ; Xiao-hua Li ; Ming-hua Duan ; Ai-ling Jia ; Ye Wang ; Da Liu ; Yi-Ping Li ; ^{czdliyiping@163.com" class="auth_mail" title="E-mail the corresponding author} ; Zhi-Dong Qiu
• 关键词：Oviductus ranae ; Oviductus ranae protein hydrolysate ; Glioma cells ; PI3K/AKT
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：81
• 期：Complete
• 页码：176-181
• 全文大小：2179 K

文摘

Oviductus Ranae is the dry oviducts of Rana temporaria chensinensis, and it has been reported to have a range of biological activities. This study aimed to investigate the effects of Oviductus Ranae protein hydrolysate (ORPH) on human glioma C₆ cell proliferation and apoptosis in vitro and in vivo. Following in vitro treatment, cell viability and colony formation assays showed that ORPH inhibited C₆ cell proliferation. In addition, the results of western blotting also demonstrated that ORPH effectively regulated the expression of the apoptosis related proteins, cleaved caspase-3, Bax and Bcl-2, DNA staining and flow cytometry analysis demonstrated that ORPH significantly promoted apoptosis in this cell line, a finding that was confirmed in vivo using terminal deoxynucleotidyl transferase dUTP nick end labeling. Further investigation demonstrated that ORPH increased apoptosis by modulating the release of inflammatory cytokines and the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway; this was demonstrated using a PI3K/AKT inhibitor (NVP-BEZ235). In summary, the present study suggested that ORPH promoted apoptosis and inhibited glioma cell proliferation by influencing the PI3 K/AKT signaling pathway.