Genetic engineering of Bacillus megaterium for high-yield production of the major teleost progestogens 17α,20β-di- and 17α,20β,21α-trihydroxy-4-pregnen-3-one
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- 作者：Adrian Gerbera ; Mohammed Milhima ; Philip Hartza ; Josef Zappb ; Rita Bernhardta ; ritabern@mx.uni-saarland.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：AKR ; aldo-keto reductase ; 17OH-P ; 17α-hydroxyprogesterone ; 17α ; 20αDiOH-P ; 17α ; 20α-dihydroxy-4-pregnen-3-one ; 17α ; 20βDiOH-P ; 17α ; 20β-dihydroxy-4-pregnen-3-one ; 20βOH-RSS ; 17α ; 20α ; 21α-trihydroxy-4-pregnen-3-one ; DOC ; 11-deoxycorticosterone ; FabG ; 3-oxoacyl-(acyl-carrier-protein) reductase ; HSD ; hydroxysteroid dehydrogenase ; LB ; lysogeny broth ; MIH ; maturation inducing hormone ; MPF ; maturation promoting factor ; NMR ; nuclear magnetic resonance ; ORF ; open reading frame ; PHB ; poly(3-hydroxybutyrate)
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：36
- 期：Complete
- 页码：19-27
- 全文大小：1323 K
文摘
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17α,20β-di- and 17α,20β,21α-trihydroxy-4-pregnen-3-one are the major fish progestogens.
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B. megaterium was genetically engineered to rapidly produce both steroid hormones.
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Innate steroid hydroxylase CYP106A1 was deleted.
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Four 20α-hydroxysteroid dehydrogenases were identified and deleted.
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B. megaterium FabG was identified as an efficient 20βHSD.