28 day continuous intravenous infusion in unrestrained mice
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文摘
This study describes a method for continuous intravenous infusion in CD-1 mice. Two treatment groups, comprising of 10 animals/sex, were surgically implanted with indwelling femoral vein catheters exteriorised via customised protective tail cuffs. The animals were continuously infused for 24 hours daily at dose volumes of 4 and 8 ml kg−1 h−1 for up to 28 consecutive days. A similarly constituted untreated group acted as cage controls. There were no adverse reactions to treatment and no changes in body weight, food and water consumption were noted for the infused animals when compared to the cage controls. Haematological data showed a slight increase in the total WBC count. Urinary volume was increased in a dose volume related manner. Histological evaluation showed mild inflammation in the femoral vein, vena cava and the tail. The results indicate that the presence of an indwelling femoral vein catheter, exteriorised via a protective tail cuff in mice was not associated with any adverse effects. Consequently, this method of administration is recommended for the continuous intravenous infusion of test solutions for up to 28 days.