Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds
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- 作者：Sergio Jarque ; Michal Bittner ; Klá ; ra Hilscherová ; ; hilscherova@recetox.muni.cz" class="auth_mail" title="E-mail the corresponding author
- 关键词：Recombinant yeast assay ; Freeze-drying ; Estrogen ; Androgen ; Long-term ; Ready-to-use
- 刊名：Chemosphere
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：148
- 期：Complete
- 页码：204-210
- 全文大小：965 K
文摘
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Freeze-drying immobilization to obtain “ready-to-use” versions of yeast biosensors.
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Immobilized yeast cells stored at −18 °C retained viability at least up to 10 months.
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Sensitivity towards androgens and estrogens was comparable to standard assays.
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The new method shortens conventional procedures from 3–4 days to 6 h in non-sterile conditions.