Highly efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by a novel carbonyl reductase from Yarrowia lipolytica and using mannitol or sorbitol as cosubstrate
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- 作者：Qin Xu ; Wei-Yi Tao ; He Huang ; Shuang Li ; lishuang@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Biocatalysis ; Carbonyl reductase ; Enzyme production ; Ethyl (S)-4-chloro-3-hydroxybutanoate ; Optimisation ; Bioconversion
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：106
- 期：Complete
- 页码：61-67
- 全文大小：991 K
文摘
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A new carbonyl reductase was discovered by genome data mining.
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10">COBE at 3000 mM was reduced to (S)-CHBE (99% e.e, yield 90%).
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15">The co-factor NADPH was recycled using sorbitol or mannitol as co-substrate.
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The proportion of co-substrate was 1.2–1.3 mmol/mmol COBE.