Isolation and characterization of a California encephalitis serogroup orthobunyavirus from Finnish mosquitoes

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• 作者：Niina Putkuri^a ; ^{niina.putkuri@helsinki.fi" class="auth_mail} ; Satu Kurkela^a ; ^b ; Lev Levanov^a ; Eili Huhtamo^a ; Antti Vaheri^a ; ^b ; Tarja Sironen^a ; ¹ ; Olli Vapalahti^a ; ^b ; ^c ; ¹
• 关键词：Orthobunyavirus ; California serogroup virus ; Mosquito-borne virus ; Chatanga virus isolate ; Snowshoe hare virus-like
• 刊名：Infection, Genetics and Evolution
• 出版年：March, 2014
• 年：2014
• 卷：22
• 期：Complete
• 页码：164-173
• 全文大小：2048 K

文摘

The mosquito-borne California encephalitis serogroup viruses of the genus Orthobunyavirus (family Bunyaviridae) include several causative agents of encephalitis in humans. Until recently, Inkoo virus (INKV) was the only orthobunyavirus isolated in Finland, showing high seroprevalence in the population. In this study, we recovered five orthobunyavirus isolates from mosquitoes collected in eastern Finland in the early autumns of 2007 and 2008 by inoculation of Vero cells. The isolates were determined by S, M and L segment sequences to represent the California encephalitis virus species but distinct from INKV (68% polyprotein amino acid (aa) identity). In genetic analyses, isolates clustered together with a number of westernmost Chatanga virus isolates (98% polyprotein aa identity) reported from Russia, forming a distinct phylogroup. However, the sequence homology of this phylogroup to the majority of Chatanga isolates, comprising three different geographically clustered phylogroups, was considerably lower (89-92% polyprotein aa identity). The five new isolates were designated as M枚hk枚 isolates of Chatanga virus, according to the village of origin. The isolates were closely related to Snowshoe hare virus (SSHV) and La Crosse virus (LACV) with an aa identity of 87% and 82% within the M segment polyprotein, respectively. The genetic relatedness of M枚hk枚 isolates to a number of human pathogenic orthobunyaviruses warrants further investigation on their potential disease associations and further serological analysis is needed to compare them to other Chatanga virus isolates and SSHV to determine their true antigenic relation.