Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools
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- 作者：Marco Schü ; tz1 ; 4 ; Alexander Batyuk1 ; Christoph Klenk1 ; Lutz Kummer1 ; 4 ; Seymour de Picciotto2 ; Basri Gü ; lbakan3 ; 5 ; Yufan Wu1 ; Gregory A. Newby1 ; 6 ; Franziska Zosel1 ; Jendrik Schö ; ppe1 ; Erik Sedlá ; k1 ; 7 ; Peer R.E. Mittl1 ; Renato Zenobi3 ; K. Dane Wittrup2 ; Andreas Plü ; ckthun1 ; plueckthun@bioc.uzh.ch" class="auth_mail" title="E-mail the corresponding author
- 关键词：DARPin ; designed ankyrin repeat protein ; nanoESI-MS ; nanoelectrospray ionization mass spectrometry ; MS ; mass spectrometry ; FACS ; fluorescence-activated cell sorting ; FAP ; fluorogen-activating protein ; GFP ; green fluorescent protein ; MG ; malachite green ; scFv ; single-chain variable fragment ; ORF ; open reading frame
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：27 March 2016
- 年：2016
- 卷：428
- 期：6
- 页码：1272-1289
- 全文大小：1743 K
文摘
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Fluorogen-activating proteins are a novel class of fluorescent probes.
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We created a fluorogen-activating DARPin (FADA) for malachite green.
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Structural investigations revealed that the fluorogen is activated by a FADA dimer.
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The FADA can be used as biosensors in extracellular and intracellular applications.
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A prototype sensor that detects a presence of another molecule has been built.