Alkaline phosphatase-fused repebody as a new format of immuno-reagent for an immunoassay

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• 作者：Hyo-Deok Seo^a ; Joong-jae Lee^a ; Yu Jung Kim^c ; Oliver Hantschel^b ; Seung-Goo Lee^c ; Hak-Sung Kim^a ; ^{hskim76@kaist.ac.kr}
• 关键词：Monomeric alkaline phosphatase ; Repebody ; Genetic fusion ; Immuno-reagent ; Immunoassay
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：950
• 期：Complete
• 页码：184-191
• 全文大小：925 K
• 卷排序：950

文摘

A human TNF-α (hTNF-α)-specific repebody was selected using a phage display. A monomeric alkaline phosphatase (mAP) was genetically fused to the repebody. mAP-fused repebody enabled detection of hTNF-α with high sensitivity and accuracy. mAP-fused repebody can be widely used as a new immuno-reagent in immunoassays.