Optimization of eGFP expression using a modified baculovirus expression system

详细信息    查看全文

• 作者：Jingping Ge ; Liying Jin ; Xiaoyan Tang ; Dongni Gao ; Qi An ; Wenxiang Ping
• 关键词：WSSV ie1 promoter ; WPRE ; ITRs ; VSV-GED ; Recombinant baculovirus
• 刊名：Journal of Biotechnology
• 出版年：10 March, 2014
• 年：2014
• 卷：173
• 期：Complete
• 页码：41-46
• 全文大小：1594 K

文摘

The baculovirus gene expression system is an efficient and safe protein expression system, since baculoviruses cannot replicate in mammalian cells. In order to improve the transduction efficiency and increase the reporter gene expression levels delivered by baculoviruses, we tested in the baculovirus expression cassette the Woodchuck hepatitis virus response element (WPRE), and AAV-derived inverted terminal repeats (ITRs) and the truncated vesicular stomatitis virus G protein (VSV-GED). The results showed that WPRE and VSV-GED have synergistic effects and could enhance the expression efficiency of enhanced green fluorescence protein (eGFP), and that ITRs effectively extended the duration of eGFP expression. We also demonstrated that the efficiency of eGFP expression varied under the control of the CMV, CBA, EF1-伪 or WSSV ie1 promoters in different cell lines.