Co-utilization of glucose and xylose by evolved Thermus thermophilus LC113 strain elucidated by 13C metabolic flux analysis and whole genome sequencing
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- 作者：Lauren T. Cordova ; Jing Lu ; Robert M. Cipolla ; Nicholas R. Sandoval ; Christopher P. Long ; Maciek R. Antoniewicz ; mranton@udel.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Thermophile ; Glucose and xylose metabolism ; Co-utilization ; Isotopic labeling ; Metabolic fluxes
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：37
- 期：Complete
- 页码：63-71
- 全文大小：1358 K
文摘
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T. thermophilus LC113 co-utilizes glucose and xylose without carbon catabolite repression.
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T. thermophilus LC113 has increased growth rate, biomass yield, and tolerance to high temperatures.
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13C-MFA revealed flexible upper carbon metabolism and a rigid lower metabolism.
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Supply of NADPH may be limiting the growth rate of T. thermophilus LC113.
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Whole genome sequencing revealed duplication/multiplication of key genetic elements.