Analysis of N′-nitrosonornicotine enantiomers in human urine by chiral stationary phase liquid chromatography-nanoelectrospray ionization-high resolution tandem mass spectrometry
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- 作者：Jing Yang ; Steven G. Carmella ; Stephen S. Hecht ; hecht002@umn.edu
- 关键词：NNN ; N&prime ; -nitrosonornicotine ; LC&ndash ; NSI&ndash ; HRMS/MS ; liquid chromatography&ndash ; nanoelectrospray ionization&ndash ; high resolution tandem mass spectrometry
- 刊名：Journal of Chromatography B
- 出版年：2017
- 出版时间：15 February 2017
- 年：2017
- 卷：1044-1045
- 期：Complete
- 页码：127-131
- 全文大小：719 K
- 卷排序：1044
文摘
An LC–NSI–HRMS/MS method was developed to quantify NNN enantiomers in human urine. [Pyridine-D4]nornicotine was used to monitor artifactual formation of NNN. (S)-NNN, the more carcinogenic enantiomer, predominated in human urine. The enantiomeric ratio in urine was remarkably similar to that in tobacco products. This is the first use of NSI-HRMS for chiral separation of urinary metabolites.