An efficient two-photon ratiometric fluorescent probe platform for dual-channel imaging of lysosomes in living cells and tissues
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- 作者：Liyi Zhoua ; zhouly0817@163.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Dan-Qing LubAuthor Vitae ; Qianqian WangbAuthor Vitae ; Shunqin HuaAuthor Vitae ; Haifei WangaAuthor Vitae ; Hongyan SuncAuthor Vitae ; Xiaobing ZhangbAuthor Vitae
- 关键词：Two-photon ; Ratiometric fluorescent probe ; Lysosomes ; Tissues ; Fluorescent imaging
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：238
- 期：Complete
- 页码：274-280
- 全文大小：2100 K
文摘
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A novel two-photon ratiometric fluorescent probe platform was developed using a FRET strategy.
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A FRET-based two-photon ratiometric fluorescent probe NRLys was developed to monitor pH change in lysosome.
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NRLys possesses two well-resolved emission peaks separated by 60 nm.
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The NRLys shows high ratiometric imaging resolution and deep-tissue imaging depth of over 180 μm.