Various responses to copper and manganese exposure of Carassius auratus gibelio from two populations

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• 作者：Halina I. Falfushynska^a ; Lesya L. Gnatyshyna^a ; Oksana B. Stoliar^a ; ^{Oksana.Stolyar@gmail.com"" rel=""nofollow} ; Yoon Kwon Nam^b
• 关键词：Carassius auratus gibelio ; Cu ; Mn ; Environmental stress ; Biochemical markers ; Metallothionein ; Oxidative damage ; Biotransformation ; Genotoxicity
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：154
• 期：3
• 页码：242-253
• 全文大小：1637 K

文摘

The aim of this study was to compare the effect of pro-oxidants copper (Cu²⁺, 0.005 and 0.050 mg L^− 1) or manganese (Mn²⁺, 0.17 and 1.7 mg L^− 1) on Carassius auratus gibelio from polluted (B) and unpolluted (Z) sites after exposure for fourteen days. Fish from site B showed high levels of lipid peroxidation (TBARS concentration), lower levels of metallothionein (MT)-related metal, total glutathione (GSH), its redox index, superoxide dismutase (Cu,Zn- and Mn-SOD), glutathione-S-transferase (GST) and cholinesterase (ChE) activities and also higher MT-related thiol concentration in the liver and gills. A common effect of exposure was related to genotoxicity, a decrease in GSH and an increase in microsomal ethoxyresorufin O-deethylase activity in the liver. However, the systems of oxidative stress and biotransformation were more efficient in fish from the polluted site, while the responsivity of MTs in this fish was impaired. Principle Component Analysis separated the subgroups from the unpolluted site and fish loaded by lesser concentrations of metals on the one side, and fish from the polluted site jointly with fish exposed to higher concentrations of metals on the other side. The main distinguishing indices of sites and exposures selected by classification and regression tree (CART) analysis were MT characteristics and genotoxicity.