Investigation of temperature-responsivity and aqueous chromatographic characteristics of a thermo-responsive monolithic column

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• 作者：Haiyan Liu ; ^a ; ^{lhy1610@126.com"" rel=""nofollow} ; Mingquan Liu^a ; Ligai Bai^a ; Suying Sun^a ; Yankun Liu^a ; Gengliang Yang ; ^a ; ^{ygl@hbu.edu.cn"" rel=""nofollow}
• 关键词：Thermo-responsive ; Cryogel ; Swelling and deswelling ; Steroids
• 刊名：Talanta
• 出版年：2011
• 出版时间：15 August 2011
• 年：2011
• 卷：85
• 期：2
• 页码：1193-1198
• 全文大小：628 K

文摘

A thermo-responsive and macroporous monolithic cryogel was directly prepared by in situ free-radical redox cryo-polymerization in a stainless steel chromatographic column (100 mm × 4.6 mm i.d.) using N-isopropylacrylamide (NIPAAm) as functional monomer and PEG-20,000 as porogen at −12 °C. The internal morphology of resulting monolithic cryogel was estimated by scanning electron microscopy (SEM). Based on that, a submicron skeleton structure was observed. Besides, the gravimetrically determined rates of swelling/deswelling for thermo-responsive monolithic cryogel were much higher than that of hydrogel adopting the same component proportion via conventional method (25 °C for 24 h). Simultaneously, a temperature-dependent resolution of steroids was also achieved using only water as a mobile phase. The theoretical plate number of every analyte was more than 2000.