Immune roles of a rhamnose-binding lectin in the colonial ascidian Botryllus schlosseri

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• 作者：Nicola Franchi^a ; Filippo Schiavon^a ; Matteo Carletto^a ; Fabio Gasparini^a ; Giulio Bertoloni^b ; Silvio C.E. Tosatto^a ; Loriano Ballarin^a ; ^{loriano.ballarin@unipd.it"" rel=""nofollow}
• 关键词：Botryllus ; Compound ascidians ; Tunicates ; Rhamnose-binding lectin ; Haemocytes
• 刊名：Immunobiology
• 出版年：2011
• 出版时间：June 2011
• 年：2011
• 卷：216
• 期：6
• 页码：725-736
• 全文大小：1434 K

文摘

The present paper describes the immune role played by a recently identified (Gasparini et al. 2008) member of the rhamnose-binding lectin (RBL) family from the colonial ascidian Botryllus schlosseri.

B. schlosseri RBL (BsRBL) can activate phagocytes through: (i) induction of their directional movement towards the source of the molecule; (ii) modification of cytoskeleton, required for shape changes; (iii) stimulation of the respiratory burst, and consequent production of reactive oxygen species (ROS) with microbicidal activity, including superoxide anions and peroxides; and (iv) increase in the ability to phagocytose foreign particles. RBL also induces the synthesis and release, by cytotoxic morula cells (MCs), of cytokines recognised by anti-IL1α and anti-TNFα antibodies. At high concentrations, BsRBL induces degranulation of MCs and the consequent release of the cytotoxic enzyme phenoloxidase into the medium. Results are consistent with the existence of cross-talk between B. schlosseri immunocytes (phagocytes and MCs). In addition, a three-dimensional model for BsRBL is presented.