Biochemical and structural characterisation of a haloalkane dehalogenase from a marine Rhodobacteraceae
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- 作者：Halina R. Novaka ; Christopher Sayera ; Michail N. Isupova ; Dorothee Gotzb ; Andrew Mearns Spraggb ; Jennifer A. Littlechilda ; J.A.Littlechild@exeter.ac.uk" class="auth_mail
- 关键词：HLD ; haloalkane dehalogenase ; l-HAD ; l-Haloacid dehalogenase ; DhlA ; Xanthobacter autotrophicus haloalkane dehalogenase ; DhaA ; Rhodococcus rhodochrous haloalkane dehalogenase ; DmbC ; Mycobacterium tuberculosis haloalkane dehalogenase ; LinB ; Sphingomonas paucimobilis haloalkane dehalogenase ; DbjA ; Bradyrhizobium japonicum USDA110 haloalkane dehalogenase ; DppA ; Plesiocystis pacifica SIR-1 haloalkane dehalogenase ; DspA ; Stronglocentrotus purouratus haloalkane dehalogenase ; HanR ; Rhodobacteraceae fami
- 刊名：FEBS Letters
- 出版年：2 May 2014
- 年：2014
- 卷：588
- 期：9
- 页码：1616-1622
- 全文大小：1559 K
文摘
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Isolation of a marine Rhodobacteraceae sp. from a tube worm.
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Identification of a putative haloalkane dehalogenase enzyme.
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Characterisation and crystal structure of the native haloalkane dehalogenase enzyme and product bound complex.
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Relationship between the substrate specificity and the size and charge distribution within the active site cavity.