Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

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• 作者：Lina Abu-Tair ; Sarit Doron ; Mahmud Mahamid ; Johnny Amer ; Rifaat Safadi
• 关键词：HSCs ; hepatic-stellate-cells ; CCl4 ; carbon-tetrachloride ; PBL ; peripheral-blood-lymphocytes ; HCV ; hepatitis-C-virus ; GSH ; reduced glutathione ; ROS ; reactive oxygen species
• 刊名：Mitochondrion
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：13
• 期：5
• 页码：473-480
• 全文大小：790 K

文摘

We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.

In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.

Leptin-receptor-deficient (db-db)-HSCs did not adhere lymphocytes. db/db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.

This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.