Phosphatidyl serine exposure during apoptosis precedes release of cytochrome c and decrease in mitochondrial transmembrane potential

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• 作者：Denecker ; Geertrui ; Dooms ; Hans ; Van Loo ; Geert ; Vercammen ; Dominique ; Grooten ; Johan ; Fiers ; Walter ; et. al.
• 关键词：Apoptosis ; Phosphatidyl serine ; Mitochondrial transmembrane potential
• 刊名：FEBS Letters
• 出版年：2000
• 出版时间：January 7, 2000
• 年：2000
• 卷：465
• 期：1
• 页码：47-52
• 全文大小：176 K

文摘

Time kinetics of phosphatidyl serine (PS) exposure were compared to other apoptotic parameters following different apoptotic stimuli. Our data indicate that anti-Fas treatment of L929sAhFas cells results in rapid exposure of PS, which precedes decrease in mitochondrial transmembrane potential (ΔΨ_m) and release of cytochrome c, indicating that PS exposure occurs independently of these mitochondrial events. Also during TNF-, etoposide- or staurosporine-mediated apoptosis in PC60 RI/RII cells, PS-positive cells were observed before they had a decreased ΔΨ_m. However, during growth factor depletion-induced death of 32D cells, both phenomena seemed to occur at the same time.