- 作者：Mariana Piana ; Mariane Arnoldi Silva ; Gabriela Trevisan ; Thiele Faccim de Brum ; C谩ssia Regina Silva ; Aline Augusti Boligon ; Sara Marchesan Oliveira ; Marina Zadra ; Carin Hoffmeister ; Mateus Fortes Rossato ; Raquel Tonello ; Luciane Varini Laporta ; Robson Borba de Freitas ; Bianca Vargas Belke ; Roberta da Silva Jesus ; Juliano Ferreira ; Margareth Linde Athayde
- 关键词：Viola tricolor ; Antiinflammatory ; Antinociceptive ; Gel stability ; Violaceae
- 刊名：Journal of Ethnopharmacology
- 出版年：25 November, 2013
- 年：2013
- 卷：150
- 期：2
- 页码：458-465
- 全文大小：1131 K
Ethnopharmacological relevance
Viola tricolor, popularly known as heartsease has been empirically used in several skin disorders, including burns.Aim of the study
The objective of this study was investigate the antinociceptive and antiinflammatory effect of a gel containing extract of Viola tricolor flowers on thermal burn induced by UVB irradiation and to perform gel stability study.
Methods
The antinociceptive and antiinflammatory effect were evaluated by static and dynamic mechanical allodynia model, paw edema, and neutrophilic cell infiltration. Metabolites compounds were quantified by HPLC. The gel stability study was performed analyzing organoleptical aspects, besides pH, viscosity, and quantification of rutin by HPLC.
Results
In the results were evidenced changes in threshold in statical and dynamic mechanical allodynia (Imax=100卤10% and 49卤10%, respectively), paw edema (Imax=61卤6%), and myeloperoxidase activity (Imax=89卤5%). Such effects may be attributed, in part, to rutin, salicylic and chlorogenic acids, and others compounds found in this species. No important changes were detected in the stability study, in all aspects analyzed in temperature below 25 掳C.
Conclusion
These findings suggest that Viola tricolor gel has an antinociceptive and antiinflammatory effect in the ultraviolet-B-induced burn, since maintain the temperature below 25 掳C.