JBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei
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- 作者：Rudo Kieft ; Verena Br ; Dilrukshi K. Ekanayake ; Kate Sweeney ; Courtney DiPaolo ; William S. Reznikoff ; Robert Sabatini
- 关键词：Trypanasoma brucei ; Base J ; DNA modification ; Antigenic variation
- 刊名：Molecular and Biochemical Parasitology
- 出版年：2007
- 出版时间：November 2007
- 年：2007
- 卷：156
- 期：1
- 页码：24-31
- 全文大小：504 K
文摘
Synthesis of the modified thymine base, β-d-glucosyl-hydroxymethyluracil or J, within telomeric DNA of Trypanosoma brucei correlates with the bloodstream form specific epigenetic silencing of telomeric variant surface glycoprotein genes involved in antigenic variation. In order to analyze the function of base J in the regulation of antigenic variation, we are characterizing the regulatory mechanism of J biosynthesis. We have recently proposed a model in which chromatin remodeling by a SWI2/SNF2-like protein (JBP2) regulates the developmental and de novo site-specific localization of J synthesis within bloodstream form trypanosome DNA. Consistent with this model, we now show that JBP2 (−/−) bloodstream form trypanosomes contain five-fold less base J and are unable to stimulate de novo J synthesis in newly generated telomeric arrays.