Determination of perfluorochemicals in biological, environmental and food samples by an automated on-line solid phase extraction ultra high performance liquid chromatography tandem mass spectrometry method

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• 作者：Fabio Gosetti ; Ugo Chiuminatto ; Davide Zampieri ; Eleonora Mazzucco ; Elisa Robotti ; Giorgio Calabrese ; Maria Carla Gennaro ; Emilio Marengo
• 关键词：Perfluorinated compounds ; On-line SPE ; UHPLC– ; MS/MS ; Blood sample ; River sample ; Food sample
• 刊名：Journal of Chromatography A
• 出版年：2010
• 出版时间：10 December 2010
• 年：2010
• 卷：1217
• 期：50
• 页码：7864-7872
• 全文大小：678 K

文摘

A rapid on-line solid phase extraction ultra high performance liquid chromatography tandem mass spectrometry method was developed for the identification and quantitation of nine perfluorinated compounds in matrices of environmental, biological and food interest. Pre-treatment, solid phase extraction, chromatographic and mass detection conditions were optimised, in order to apply the whole methodology to the analysis of different matrices. Particular attention was devoted to the evaluation of matrix effect and the correlated phenomena of ion enhancement or suppression in mass spectrometry detection. LOD and LOQ range from 3 to 15 ng L⁻¹ and from 10 to 50 ng L⁻¹, respectively. Method detection limits (MDLs) were also calculated for each kind of matrix. The recovery, evaluated for each analyte, does not depend on analyte concentration in the explored concentration range: average values are always greater than 82.9 % . In the whole, the results obtained for samples of river waters, blood serum, blood plasma, and fish confirm the ubiquitous presence of perfluorinated compounds, as recently denounced by many sources.