Diversity of polymyxin resistance mechanisms among Acinetobacter baumannii clinical isolates

详细信息    查看全文

• 作者：Raquel Girardello^a ; ^{raquelgirardello@gmail.com} ; Marina Visconde^a ; Rodrigo Cay&ocirc ; ^a ; Regina Cé ; lia Bressan Queiroz de Figueiredo^b ; Marcelo Alves da Silva Mori^c ; Nilton Lincopan^d ; ^e ; Ana Cristina Gales^a
• 关键词：A. baumannii ; Polymyxin resistance ; Virulence
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：87
• 期：1
• 页码：37-44
• 全文大小：1132 K
• 卷排序：87

文摘

Our results showed that the mechanisms of resistance to polymyxin B varied significantly between A. baumannii clinical isolates. In contrast to previously reported data, the A027ind strain had acquired simultaneously mutations in both pmrB and lpxA genes after polymyxin B exposure. By transmission electronic microscopy, the exposure to polymyxin B induced a strong condensation and darkening of intracellular material of A. baumannii isolates. The C. elegans assays showed that the A027 strain, which was initially susceptible to polymyxin B, was more virulent than polymixin-resistant A009 strain. However, after polymyxin B exposure, A027ind strain displayed a significant decrease of virulence, which was not observed in the A009ind strain.