Characterization of a new variant DNA (cytosine-5)-methyltransferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus

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• 作者：del Gaudio ; Rosanna ; Di Giaimo ; Rossella ; Potenza ; Nicoletta ; Branno ; Margherita ; Aniello ; Francesco ; et. al.
• 关键词：DNA methyltransferase ; Invertebrate ; Annelid polychaete ; 5-Methylcytosine ; Chaetopterus variopedatus
• 刊名：FEBS Letters
• 出版年：1999
• 出版时间：October 29, 1999
• 年：1999
• 卷：460
• 期：2
• 页码：380-384
• 全文大小：86.5 K

文摘

The enzyme S-adenosylmethionine-DNA (cytosine-5)-methyltransferase has been identified, first time for invertebrates, in embryos of the marine polychaete annelid worm Chaetopterus variopedatus. The molecule has been isolated from embryos at 15 h of development. It is a single peptide of about 200 kDa molecular weight, cross-reacting with antibodies against sea urchin DNA methyltransferase. The enzymatic properties of the molecule are similar to those of Dnmt1 methyltransferases isolated from other organisms, but with the peculiarity to be unable to make ‘de novo’ methylation on double stranded DNA.