Genotyping of five short tandem repeat loci via triplex and duplex PCR

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• 作者：Rostedt ; Irma ; Lalu ; Kaisa ; Lukka ; Matti ; Sajantila ; Antti
• 关键词：Finnish population ; Multiplex PCR ; Paternity testing ; Short tandem repeats
• 刊名：Forensic Science International
• 出版年：1996
• 出版时间：October 25, 1996
• 年：1996
• 卷：82
• 期：3
• 页码：217-226
• 全文大小：813 K

文摘

We have developed a triplex PCR method for D3S1359, HumTH01 and HumTPO tetranucleotide loci and a duplex PCR method for HumFES/FPS and HumvWA31A tetranucleotide loci using high resolution polyacrylamide gel electrophoresis and silver staining. The methods were evaluated for paternity testing and individual identification and allele frequencies at these loci are reported for 189-3387 unrelated individuals in the Finnish population. The D3S1359 locus, especially, was found to be a highly informative locus. Seventeen alleles were found in the D3S1359 locus with a highest observed allele frequency of 0.199, a high exclusion power (PE) in paternity testing (0.78) and a high observed heterozygosity (0.89). The combined PE for these five loci was 0.99.