Effect of Maternal Immune Status on Responsiveness of Bacillus Calmette-Gu¨¦rin Vaccination in Mouse Neonates

详细信息    查看全文

• 作者：Jong Su Choi ; Ryang Yeo Kim ; Semi Rho ; Fanny Ewann ; Nathalie Mielcarek ; Man Ki Song ; Cecil Czerkinsky ; Jae-Ouk Kim
• 关键词：BCG ; maternal effect ; Mycobacterium tuberculosis ; neonates ; tuberculosis
• 刊名：Osong Public Health and Research Perspectives
• 出版年：2012
• 出版时间：June, 2012
• 年：2012
• 卷：3
• 期：2
• 页码：68-73
• 全文大小：375 K

文摘

Objectives

Bacillus Calmette-Gu¨¦rin (BCG) vaccination has proven to be efficient in immunologically na?ve infants; however, it has not been investigated that maternal natural exposure to Mycobacterium and/or BCG vaccine could influence the characteristics of immune responses to BCG in newborns. In this study, we analyzed whether the maternal immune status to M tuberculosis (M tb) can affect neonatal immunity to BCG using a mouse model.

Methods

Neonates were obtained from mice that were previously exposed to live BCG, to live M avium, or to heat-killed M tb H37Rv, and from na?ve control mothers. One week after birth, the neonates were divided into two subgroups: one group immunized with live BCG via the subcutaneous route and the other group of neonates sham-treated. Interferon-gamma (IFN¦Ã) secretion in response to in?vitro stimulation with heat-killed BCG or purified protein derivative (PPD) was examined. Protection against M tb infection was evaluated by challenging mice nasally with live M tb H37Rv followed by counting colonies from spleen and lung homogenates.

Results

BCG-immunized neonates showed increased IFN¦Ã secretion in response to heat-killed BCG or PPD. All mice in BCG-immunized neonates subgroups showed reduced bacterial burden (colony forming unit) in the lungs when compared with control naive neonate mice. However, no statistically significant difference was observed when comparing BCG-immunized mice born from mothers previously exposed to M avium or immunized with either heat-killed H37Rv or live BCG and mice born from na?ve mothers.

Conclusion

The maternal immune status to M tb does not appear to impact on the immunogenicity of BCG vaccine in their progeny in our experimental conditions.