Comparative investigation of the free radical scavenging potential and anticancer property of Diospyros blancoi (Ebenaceae)

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• 作者：Muhammad Ali Khan¹ ; Md. Mahbubur Rahman² ; Md. Nazmul Sardar² ; Md. Saiful Islam Arman² ; Md. Badrul Islam³ ; Md. Jahangir Alam Khandakar⁴ ; Mamunur Rashid² ; Golam Sadik² ; AHM Khurshid Alam² ; ⁵ ; ^{khurshid.jaist@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Diospyros blancoi ; Free radicals ; Polyphenols ; Antioxidant ; Cytotoxicity ; Anticancer
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：6
• 期：5
• 页码：410-417
• 全文大小：895 K

文摘

To investigate the comparative effects of Diospyros blancoi (Ebenaceae) leaves (DBL), root bark (DBRB) and stem bark (DBSB) on free radicals and cancer.

Methods

The polyphenol contents, antioxidant and free radical scavenging properties were determined using standard spectrophotometric methods. Cytotoxicity and anticancer activities were performed on brine shrimp nauplii and Ehrlich ascite carcinoma cells, respectively.

Results

Among the extracts, DBSB showed the highest total antioxidant capacity and reducing capacity on ferrous ion. Based on 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical scavenging activities, DBSB showed (95.760 ± 0.343)% and (67.460 ± 2.641)% scavenging with IC₅₀ of (3.10 ± 0.17) and (50.00 ± 3.11) μg/mL, respectively. The IC₅₀ values of standard butylated hydroxytoluene and catechin (CA) for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals were (8.50 ± 0.25) and (75.00 ± 0.14) μg/mL, respectively suggesting that DBSB had a significant (P < 0.05) radical scavenging activity than standards. In lipid peroxidation inhibition assay, the inhibitory activity of the extracts and the standard was in the following order: DBSB > DBRB > CA > DBL. Also, the phenolic [(139.91 ± 3.924) mg gallic acid equivalent/g] and flavonoid contents [(412.00 ± 16.70) mg catechin equivalent/g)] of DBSB were higher than that of other extracts. In addition, the DBSB showed the moderate cytotoxic and anticancer properties.

Conclusions

Our results indicate that Diospyros blancoi stem bark had the significant highest antioxidant and free radical scavenging properties as well as moderate anticancer activity. Hence, we assume that the anticancer activity of this plant can be, at least in part, attributed to its content in phenolic compounds as well as its significant free radical scavenging properties.