Bisphenol A 3,4-quinone induces the conversion of xanthine dehydrogenase into oxidase in vitro

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• 作者：Satoru Sakuma ; Masahiko Nakanishi ; Kazuhiro Morinaga ; Mihoyo Fujitake ; Shun-ichi Wada ; Yohko Fujimoto
• 关键词：sphenol A 3 ; 4-quinone ; Bisphenol A ; Xanthine oxidase ; Xanthine dehydrogenase ; Reactive oxygen species ; Oxidative injury
• 刊名：Food and Chemical Toxicology
• 出版年：2010
• 出版时间：August-September 2010
• 年：2010
• 卷：48
• 期：8-9
• 页码：2217-2222
• 全文大小：681 K

文摘

In the present study, we assessed the influence of bisphenol A (BPA) and bisphenol A 3,4-quinone (BPAQ) on the conversion of xanthine dehydrogenase (XD) into xanthine oxidase (XO) in the rat liver in vitro. BPA up to 100 μmol/L did not affect the XO and XD activities in the partially purified cytosolic fraction from rat liver, whereas BPAQ (2–10 μmol/L) dose-dependently enhanced the XO activity concomitant with a decrease in the XD activity, implying that BPAQ, but not BPA, can convert XD into the reactive oxygen species (ROS) producing the form XO. Furthermore, it was found that BPAQ could increase the generation of ROS and oxidize the guanine moiety of deoxyguanosine in the DNA of primary rat hepatocyte cultures. These results suggest that BPAQ has the potential to convert XD into XO in the liver, which in turn may lead to ROS generation and oxidative DNA damage in this region.