文摘
We assessed 14 strains within four species of the marine chlorophyte genus, Dunaliella for their potential utility in sustainable biofuel production by tracking lipid production under salinity stress. High salinity stress generally resulted in maximal total fatty acid (FA) content (up to 65% by dry weight) in comparison to controls (~ 10–25% total FAs by dry weight). The unique strain, Dunaliella viridis UTEX ZZ1150, was most promising for lipid production because it produced high amounts of lipids throughout its growth cycle. Glycerol production increased significantly 30 min to 24 h after exposure to high salinity, depending on the strain, and a primary glycerol biosynthesis gene, glyceryl-3-phosphate dehydrogenase (GDPH), was significantly expressed 30 min after exposure and continued to be expressed for 24 h. Glycerol apparently acted not only as an osmolyte during hyperosmotic stress, but also provided the carbon structure to which fatty acids were covalently linked to form neutral lipids.