Environmental stressors and lipid production in Dunaliella spp. II. Nutrients, pH, and light under optimal or low salinity

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• 作者：Stephanie Mixson Byrd ; ^{steph.mixson.bird@gmail.com} ; JoAnn M. Burkholder ^{jburk@ncsu.edu}
• 关键词：FAs ; Fatty acids ; FID ; Flame ionization detector ; GC ; Gas chromatography ; NR ; Nile Red ; TAGs ; Triacylglycerides
• 刊名：Journal of Experimental Marine Biology and Ecology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：487
• 期：Complete
• 页码：33-44
• 全文大小：1241 K
• 卷排序：487

文摘

Fourteen strains within four species of the halophytic microalgal genus, Dunaliella were rapidly screened for cellular lipid content during senescence using an adapted Nile Red technique. Four promising strains that exhibited both rapid population growth and high lipid content, including three strains of Dunaliella tertiolecta and one strain of D. viridis, were selected to attempt to enhance lipid production as total fatty acids (FAs) under environmental stressors including nutrient limitation or deprivation of inorganic nitrogen (nitrate, N) and/or phosphorus (P), pH stress (control - early senescent cultures, versus pH 10 or pH 7), a 12-h: 12-h light:dark (L:D) photoperiod versus continuous light, and enrichment versus no enrichment with carbon dioxide (± 4-hr pulse daily of bubbling with CO2). All but one strain had minimal lipid production during active growth, and increased lipid production under senescence. The unique strain Dunaliella viridis UTEX ZZ1150 produced high amounts of total FAs during active growth as well as senescence, whether in a 12:12 L:D photoperiod or under continuous light. High pH × low salinity, low pH (7), and continuous light + pulsed CO2 yielded the maximum total FA content of 56%, 43%, and 42% (dry weight basis), respectively, in comparison to controls (~ 10 to 25% total FAs). Low N and/or P under a 12:12 L:D photoperiod or continuous light, and a 12:12 L:D with pulsed CO2, did not significantly enhance total FA production.