Occurrence of extended-spectrum 尾-lactamases among isolates of Salmonella enterica subsp. enterica from food-producing animals and food products, in Portugal

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• 作者：Lurdes Clemente ; Vera Manageiro ; Eug茅nia Ferreira ; Daniela Jones-Dias ; Ivone Correia ; Patr铆cia Themudo ; Teresa Albuquerque ; Manuela Cani莽a
• 关键词：Salmonella enterica ; Food-producing animals ; ESBL ; CTX-M group ; Multidrug resistance
• 刊名：International Journal of Food Microbiology
• 出版年：15 October, 2013
• 年：2013
• 卷：167
• 期：2
• 页码：221-228
• 全文大小：221 K

文摘

A total of 1120 Salmonella spp. isolates, recovered from poultry, swine and food products of animal origin (bovine, swine and poultry) over the period of 2009-2011, were investigated in order to determine their serotype, susceptibility to a panel of eleven antimicrobials (A, ampicillin; Ct, cefotaxime; Cp, ciprofloxacin; Tm, trimethoprim; Su, sulfamethoxazole; C, chloramphenicol; S, streptomycin; G, gentamicin; T, tetracycline; NA, nalidixic acid; Fl, florfenicol), and the presence of resistance determinants of extended-spectrum cephalosporins. Overall, Salmonella Enteritidis was the most common serotype in all three animal species. In 618 isolates of poultry, 32.8% comprised S. Enteritidis, 18.3% Salmonella Havana and 16.5% Salmonella Mbandaka; in 101 isolates of pigs, 21.8% comprised Salmonella Rissen and Salmonella Typhimurium, 10.9% Salmonella Derby and Salmonella London. Salmonella I 4,[5],12:i:- was the most common serotype recovered from pork and beef food products comprising 32.6% and 30% of isolates respectively, followed by S. Rissen (26% and 24%) and S. Typhimurium (18.2% and 19%), respectively. In poultry products, S. Enteritidis was the most frequent serotype (62.7%), followed by S. Mbandaka (10.2%) and S. Derby (8.5%). Susceptibility profiles differed according to the origin of the isolates. Five multidrug resistant isolates (0.45%) were further characterized as extended-spectrum 尾-lactamase (ESBL) producers. Polymerase chain reaction and sequencing of the amplicons confirmed the presence of bla_CTX-M-1 (n = 2), bla_CTX-M-14 (n = 1), bla_CTX-M-15 (n = 1) and bla_CTX-M-32 (n = 1); bla_SHV-12 and bla_TEM-1 genes were also detected in two isolates of S. I 4,[5],12:i:-. Four isolates, two S. Havana and two S. I 4,[5],12:i:-, carried class 1 integrons and in three, two S. I 4,[5],12:i:- and one S. Havana, ISEcp1 was identified associated to bla_CTX-M-1, bla_CTX-M-32 and bla_CTX-M-14 genes. Additionally, in one S. I 4,[5],12:i:- isolate, orf477 was identified linked to bla_CTX-M-32. No plasmid mediated quinolone resistance-encoding genes were detected. Here, we report for the first time the presence of bla_CTX-M genes in Salmonella enterica subsp enterica isolates recovered from poultry and food products of swine origin, in Portugal.